Figure 2.

CCL5 Accelerates Hematopoietic Regeneration following Ionizing Radiation

(A) Total cells following 300 cGy and culture with 30 ng/mL CCL5 + TSF compared with TSF alone at 72 h. n = 3 per group, ^∗p = 0.0002.

(B) Total KSL cells at 72 h after 300 cGy in culture with TSF + CCL5 compared with TSF alone. n = 3 per group, ^∗p = 0.0002.

(C) Left, CFCs of KSL and progeny at 72 h and right, CFCs at 7 days following 300 cGy and culture with CCL5 + TSF or TSF alone. n = 3 per group, ^∗p = 0.008 and p = 0.007 for 72 h and 7 days, respectively.

(D) Schematic diagram of study. C57BL/6 mice were exposed to 500 cGy. 24 h after irradiation, mice were injected subcutaneously with 0.1 μg/g body weight CCL5 or saline from day 1 to 5. On day 7, marrow was collected for hematopoietic assays.

(E) Left, H&E staining of marrow at day 7. Scale bar, 100 μm. Right, bone marrow cell counts per femur. n = 11–18 per group, ^∗p = 0.004.

(F) Total SLAM + KSL cells per femur. n = 5–10 per group, ^∗p = 0.03.

(G) CFU-S12 on day 7 following 500 cGy and treatment with CCL5 or saline. n = 3 per group, ^∗p = 0.03.

(H) Levels of LTC-ICs on day 7 following 500 cGy and treatment with CCL5 or saline. n = 4–5 per group. ^∗p = 0.002.

(I) Survival following 700 cGy TBI and subcutaneous injections given 3 doses of 0.1 μg/g body weight CCL5 or saline per week from days 1 to 15. n = 14–40 mice per group. ^∗p = 0.03 by log rank analysis. Four of 40 mice were alive in the saline-treated group compared with 6 of 14 mice alive in the CCL5-treated group at day 25. The median survival for saline-treated and CCL5-treated mice are 16 and 19.5 days, respectively. Data are shown as means ± SEM. Student's t test (two-tailed with unequal variance) was applied to the data except for survival analysis where log rank analysis was applied.