Figure 3.
CCR5 Is Necessary for Hematopoietic Regeneration In Vitro and In Vivo
(A) mRNA expression of Ccr5 in peripheral blood (PB) or bone marrow (BM) in Ccr5−/− compared with Ccr5+/+ control mice. n = 6–30 per group. n.d., not detected. Data are normalized to Ccr5+/+ control samples and GAPDH.
(B) Total cells at 72 h or day 7 of 300-cGy irradiated BM KSL cells from Ccr5−/− or Ccr5+/+ mice. n = 5–6 per group, ∗p = 0.004 and p = 0.03 for 72 h and day 7, respectively.
(C) CFCs per 2 × 103 KSL and progeny cells at 72 h or day 7. n = 6 per group, ∗p = 0.004 and p < 0.0001 for 72 h and day 7, respectively.
(D) Schematic diagram of study. Ccr5−/− or control Ccr5+/+ mice were exposed to 500 cGy. Hematopoietic assays were performed on day 7 postirradiation.
(E) H&E-stained femurs from Ccr5−/− mice at day 7 following 500 cGy compared with Ccr5+/+ mice. Scale bars, 500 μm (top) and 100 μm (bottom). Quantification of total cells per femur. n = 6–9 per group, ∗p = 0.03.
(F) Left, representative flow cytometric analysis from Lin− gate of KSL cells on day 7 from Ccr5−/− and Ccr5+/+ mice irradiated with 500 cGy. Nonirradiated wild-type control is shown to left. Right, quantification of KSL cells per femur. n = 8–9 per group, ∗p = 0.01.
(G) Levels of CFCs and CFU-S12 on day 7 following 500 cGy of Ccr5−/− and Ccr5+/+ mice. n = 3–12 per group. ∗p = 0.002 and p = 0.005 for CFCs and CFU-S12, respectively. Data are shown as means ± SEM. Student's t test (two-tailed with unequal variance) was applied to the data.