Figure 4.

CCR5 Is Necessary for Hematopoietic Regeneration

(A) Schematic diagram of irradiation of Ccr5^+/+ or Ccr5^−/− mice and transplantation of 10⁶ donor cells and 2 × 10⁵ competing host marrow cells into lethally irradiated B6.SJL recipient mice.

(B) Representative flow cytometric analysis of total donor bone marrow engraftment at 16 weeks posttransplantation.

(C) Percentage of total donor CD45.2⁺ cell, myeloid (Mac1/Gr-1), T cell (CD3), and B cell (B220) engraftment in the bone marrow of CD45.1 recipient mice at 16 weeks following transplantation. n = 9 per group, ^∗p = 0.04 for total engraftment and Mac1/Gr-1, respectively.

(D) Representative flow cytometric analysis of CD45.2⁺ donor cells within bone marrow KSL cells at 16 weeks.

(E) Quantification of percentage KSL and total KSL cells per femur. n = 9 per group, ^∗p = 0.002 and p = 0.0007 for percentage KSL cells and total KSL cells, respectively.

(F) The mean percentages of CD45.2 donor cells within bone marrow KSL cells. n = 9 per group, ^∗p = 0.04. Data are shown as means ± SEM. Student's t test (two-tailed with unequal variance) was applied to the data.