Figure 5.

Deletion of Ccr5 in Hematopoietic Cells Delays Hematopoietic Regeneration

(A) Schematic diagram of isolation of Ccr5 deficiency to the hematopoietic compartment. B6.SJL (CD45.1) recipient mice were irradiated with 950 cGy and then transplanted with 5 × 10⁶ WBM cells from Ccr5^+/+ or Ccr5^−/− mice. At 12 weeks posttransplantation, donor chimerism was measured. Hematopoietic progenitor assays were performed on day 7 following 500 cGy TBI.

(B) Levels of peripheral blood donor engraftment by flow cytometric analysis in Ccr5^+/+ HC or Ccr5^−/− HC mice. n = 15 per group. Levels of mean engraftment were >97%.

(C) RT-PCR of Ccr5 mRNA expression of hematopoietic cells that are CD45⁺ and negative for mouse endothelial cell antigen (MECA). n.d., not detected. n = 3 per group. Data are normalized to Ccr5^+/+ HC and GAPDH.

(D) H&E staining of femurs on day 7 following 500 cGy TBI. Scale bars, 100 μm.

(E–G) Bone marrow cell counts (E), total KSL cells per femur (F), and CFCs (G) per 4 × 10⁴ WBM cells in Ccr5^+/+ HC and Ccr5^−/− HC at day 7 following 500 cGy TBI. n = 6–8 per group. ^∗p = 0.03, ^∗p = 0.007, and ^∗p = 0.01 for total cells per femur, total KSL cells per femur, and CFCs, respectively. Data are shown as means ± SEM. Student's t test (two-tailed with unequal variance) was applied to the data.