Figure 1.

Animal J02370 Gene Marking in Peripheral Blood, Subsets, and HSPC Colonies

At the indicated days following transplantation with autologous, lentivirus-transduced CD34⁺ HSPCs, peripheral blood draws were collected from animal ID J02370.

(A) Percent of granulocytes and lymphocytes expressing GFP. Arrows indicate time points at which O⁶BG/BCNU chemotherapy was applied.

(B) GFP expression in peripheral blood CD20⁺ and bone marrow CD34⁺ subsets. Preselection samples were collected on day 174 posttransplant, while postselection samples were collected on days 3,380 (CD20⁺ PBMC) or 3,424 (CD34⁺ BM) posttransplant.

(C) Bone marrow-derived total leukocytes from the indicated long bones were plated in colony-forming assays. Gene marking was measured by counting the proportion of GFP⁺ colonies out of 100 total.