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. 2019 Jul 8;12:5395–5404. doi: 10.2147/OTT.S208637

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© 2019 Li et al.

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Lys-trimethylation was observed in different proteins in kidney-derived cells and tissues.

Notes: (A) The levels of Lys-trimethylation in HEK293T and 786-0 cells were quantified by Western blotting; (B) The levels of Lys-trimethylation in kidney cancer tissues and adjacent normal renal cortex tissues were quantified by Western blotting. Tubulin served as the loading control. Marker: protein marker (Fermentas, USA, Catalog # 26616-ladder-002); The targeted proteins were stained with fluorescence-conjugated secondary antibodies (IRDye800CW or IRDye680 conjugated IgG, LICOR).

Abbreviations: 293T, HEK293T cells; 7860, 786-0 cells; A, adjacent normal renal cortex tissues; T, kidney cancer tissues.