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. 2019 Jul 8;12:5395–5404. doi: 10.2147/OTT.S208637

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© 2019 Li et al.

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LDHA is Lys-trimethylated in HEK293T cells.

Notes: (A) HEK293T and 786–0 cell lysates were immunoprecipitated with anti-trimethyl lysine antibodies, and the proteins in these lysates were separated by SDS-PAGE and stained using Coomassie Blue; (B) The protein bands on the SDS-PAGE gel stained notably with Coomassie Blue were cut off based on the molecular weight markers used and analyzed by LC-MS/MS. The results of the MASCOT and SEQUEST analyses indicated that LDHA was Lys-trimethylated on the peptides comprising the ATLKDQLIYNLLKEEQTPQNK sequence.

Abbreviations: 293T, HEK293T cells; 7860, 786–0 cells.