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. 2019 Jul 8;12:5377–5388. doi: 10.2147/OTT.S202026

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© 2019 Hu et al.

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Effect of lycorine on the proliferation and colony formation of OS cells.

Notes: (A) The anti-proliferative effect of lycorine on OS cell lines was determined by CCK8 assay. Cells were treated with different concentrations of lycorine for 24, 48, and 72 h. (B) Comparison of the effect of lycorine on human bone marrow stromal cells with that on OS cells for 24 h. (C) EdU assay detecting cell proliferation. EdU assays were performed 48 h after treatment with lycorine at various concentrations. (D) EdU-positive cells were caculated with the following formula: (EdU add-in cells/Hoechst stained cells) ×100%. (E) Representative images of the colony formation assays of MNNG/HOS and U2OS cells are shown. (F, G) The colonies that consisted of at least 50 cells were counted. Data are presented as the mean ± SD of three independent experiments. **P<0.001 vs control.

Abbreviations: OS, osteosarcoma; EdU, 5-ethynyl-2-deoxyuridine.