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Design of a padlock probe exemplified by the Amanita bisporigera probe (Ab-PLP). Linker regions are in bold letters, and HRCA primer binding sites are in bold capital letters. Species-specific sites of the HRCA padlock probe were plain and capitalized. The line of dots indicates connection of adjacent bases, and the numbers 496 and 557 indicate the positions in the ITS region of A. bisporigera.
