Table 2.
The information of primers and padlocks used for amplification.
| Name | Type | Sequence (5′→3′) | Length (bp) |
|---|---|---|---|
| Ab-primers | F3 | GAGGAGCATGCCTGTTTG | 18 |
| B3 | GGTCAGACAGTTAGGGTTAG | 20 | |
| FIP | CCTGCAACTCCCAAAATCCAtaatGTCATTAACATCTCAAGACCTG | 46 | |
| BIP | TTAGTGGAGAAAAGCTGTTGAACTCtataACAGCAGAGACAACTCGACG | 49 | |
| LB | AAATCTATCAATGCCAGGAGCAA | 23 | |
| Ae-primers | F3 | AATCTTTGAACGCACCTTG | 19 |
| B3 | GACAGTTAGACAGCAGAGA | 19 | |
| FIP | ACCCCCAAAATCCAATACCTATCAAtaatGAGCATGCCTGTTTGAGT | 47 | |
| BIP | GTGGAGAAAAAGCCATTTGAACTCCtataAACTAGCATTGCTCCTGG | 47 | |
| LF | TCAGACAGGTCTTGAGACTTTAATG | 25 | |
| Af-primers | F3 | TGCCTGTTTGAGTGTCATT | 19 |
| B3 | GGACATGGATTAGACAGCA | 19 | |
| FIP | GCTATACAAGCCCTGCAACCtaatAACATCTCAATACCTGTCTGC | 45 | |
| BIP | AGAAAGCTCATTGAACTCCATTGGtataAACTTGGACATTGTTCCTGG | 48 | |
| LF | CCCAATTTCCAATACCCATCAA | 22 | |
| Apa-primers | F3 | CATGCCTGTTTGAGTGTC | 18 |
| B3 | GTCAAGTGGGTCAGACAG | 18 | |
| FIP | GAAACAGCCTGCAACTCCCAtaatATTAACACCTCAAGACCTGTC | 45 | |
| BIP | TTAGTGGAGAAAAGCTGTTGAACTCtataGCAGAGACCACTTGATGTT | 48 | |
| LB | GATAAAATCTATCAATGCCAGGAGC | 25 | |
| Aph-primers | F3 | GCCTTGCTCTCTTTGAATGT | 20 |
| B3 | GATATGCTTAAGTTCAGCGG | 20 | |
| FIP | AGTGATATTGCTCCTGGCATTGtaatGAGAAAAGCCATTGAACTCC | 46 | |
| BIP | TGCTGTCTAACTGTGACTGTCTtataTAGTCCTACCTGATTTGAGGT | 47 | |
| LB | TGGATGGGGACAACTTGACC | 20 | |
| Ar-primers | F3 | TGCCTGTTTGAGTGTCATT | 19 |
| B3 | AGTTGGTCAAGTTGTCCAT | 19 | |
| FIP | CATTCAAGGAGAGCAAGCTATTTGAtaatGTCTGCTTTTGATAGGTTTCG | 50 | |
| BIP | GGTGTGATAAAATGGTATCAATGCCtataCTACAGACAGTTAGCTGAGA | 49 | |
| LF | CAACCTGCAACCCCCATAAATC | 22 | |
| Asubf-primers | F3 | TGCCTGTTTGAGTGTCATT | 19 |
| B3 | GGGTTAGACAGCAGAGAGA | 19 | |
| FIP | GCAAGCCATTTAGAAATAGCCTGCtaatGACCTGTCTGCTTTTGGA | 46 | |
| BIP | TTAGTGGAAAAAGCCATTGAACTCCtataGCTGATCATTGCTCCTGG | 47 | |
| LF | ACCCCCAAATTCCAATACCCA | 21 | |
| Asubj-primers | F3 | GCCTTGCTCTCCTTGAATGT | 20 |
| B3 | GATATGCTTAAGTTCAGCGG | 20 | |
| FIP | GTAGTGATATTGCTCCTGGCATtaatGAAAAGCCATTGAACTCCAT | 46 | |
| BIP | TGCTGTCTAACTGTGACTGTCTtataTAGTCCTACCTGATTTGAGGT | 47 | |
| LB | GGATGGGGACAACTTGACCAAC | 22 | |
| Asubp-primers | F3 | GATTTTTGGGTGTTGCA | 17 |
| B3 | AGGTCAAGTTGGTCAAGTT | 19 | |
| FIP | CCAATGGAGTTCAATGGCTCTTCtaatGCTTTTTCAGATAGCTTGCT | 47 | |
| BIP | TCTATCAATGCCAGGAGCCtataTTACAGACAACTGTGAGA | 41 | |
| LB | ATGTTAGTTCTCTCTGCTGTC | 21 | |
| Av-primers | F3 | CATCTCAAGACCTGTCTGTT | 20 |
| B3 | AGTTGGTCAAGTTGTCCAT | 19 | |
| FIP | GAGTTCAATGGCTCTTTCTCCACTAtaatGGATTTTTGGGGGTTTGC | 47 | |
| BIP | TCTATCAATGCCCAGGAGCCtataAGACAACTGTTAGCGGTTAG | 44 | |
| LF | CATTCAAGGAGAGCAAGCTATCTG | 24 | |
| Universal primers | F3 | GCAGAATTCAGTGAATCATC | 20 |
| B3 | TTGCTCCTGGCATTGATA | 18 | |
| FIP | TGACACTCAAACAGGCATGCtaatAATCTTTGAACGCACCTTG | 43 | |
| BIP | CTTGCTCTCCTTGAATGTATTAGTtataGATTTTATCACACCAATGGAGTT | 51 | |
| LF | TCCTCGGAATGCCAAGGAG | 19 | |
| Ab-PLP | P-GACGTTGCTCCTGGCATTGATATTCCCAATAGGTCCAGAATGTCAGCCGTTCCT CACACCAGACTGCCCTGAGAAATAATCTAAGATTAGACAGCAGAGACAACTC | 106 | |
| Ae-PLP | P-CATTGCTCCTGGCATTGATAGATTTTCCCAATAGGTCCAGAATGTCAGCCGTTCCTC ACACCAGACTGCCCTGAGAAATAATCTAAGAGTTAGACAGCAGAGATAACTAG | 110 | |
| Af-PLP | P-CCTGCAACCCCCAATTTCCATTCCCAATAGGTCCAGAATGTCAGCCGTTCC TCACACCAGACTGCCCTGAGAAATAATC TAAGAAGAGCAAAGCTATACAAGC | 103 | |
| Apa-PLP | P-ACTTGATGTTGCTCCTGGCATTGATTTCCCAATAGGTCCAGAATGTCAGCCGTTCCT CACACCAGACTGCCCTGAGAAATAATCTAAGAGGTTAGACAGCAGAGACC | 107 | |
| Aph-PLP | P-TTATTTGAAACAGCCTGCAACCCTTCCCAATAGGTCCAGAATGTCA GCCGTTCCTCACACCAGACTGCCCTGAGAAATAATCTAAGAAAGAGAGCAAGGCTATTT | 105 | |
| Ar-PLP | P-TTGTTCATTGCTCCTGGCATTGATATTCCCAATAGGTCCAGAATGTCAGCCGTTCCT CACACCAGACTGCCCTGAGAAATAATCTAAGAACAGTTAGCTGAGAGAACTG | 109 | |
| Asubf-PLP | P-TCAAGAGACCAGTCAAAAGTCTCTCATTTCCCAATAGGTCCAGA ATGTCAGCCGTTCCTCACACCAGACTGCCCTGAGAAATA ATCTAAGAGATTCCAATTCAAATCAAT | 110 | |
| Asubj-PLP | P-AGTGATATTGCTCCTGGCATTGATATTCCCAATAGGTCCAGAATGTCAGCCGTTCCTCACA CCAGACTGCCCTGAGAAATAATCTAAGAGTTAGACAGCAGAGAGAAGT | 109 | |
| Asubp-PLP | P-GTTAGACAGCAGAGAGAACTAACATGGCTTCCCAATAGGTCCAG AATGTCAGCCGTTCCTCACACCAGACTGCCCTGAGAAATA ATCTAAGATTTTACAGACAACTGTGAGA | 112 | |
| Av-PLP | P-GTTAGACAGCAGAGAGAACTAACATGGCTTCCCAATAGGTCCAGAATGTCAGCCGTT CCTCACACCAG ACTGCCCTGAGAAATAATCTAAGATTACAGACAACTGTTAGCG | 111 | |
| HRCA-primer 1 | GTGAGGAACGGCTGACATTCTG | 22 | |
| HRCA-primer 2 | ACCAGACTGCCCTGAGAAATAAT | 23 |
HRCA, hyperbranched rolling circle amplification; PLP, padlock probe; Ab, A. bisporigera; Ae, A. exitialis; Af, A. fuliginea; Apa, A. pallidorosea; Aph, A. phalloides; Ar, A. rimosa; Asubf, A. subfuliginea; Asubj, A. subjunquillea; Ab, A. subpallidorosea; Av, A. virosa. taat, tata were the spacers between F1c and F2, B1c and B2, respectively. P represents phosphorylation and bold represents the 5′ and 3′ arms of the PLP matched to the target region.