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. 2019 Jul 2;10:1415. doi: 10.3389/fmicb.2019.01415

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Copyright © 2019 Wang, Cheng, Xiao, Cheng, Fu, Chen, Jiang and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biological properties of four individual strains: SZ-150/A6, SZ-150/R59, SZ-150/R6, and SZ-150. (A) Detection of mycoviruses Sclerotinia sclerotiorum hypovirus 1 (SsHV1/SZ-150) and its related satellite RNA (SatH), Sclerotinia sclerotiorum mycotymovirus 1 (SsMTV1/SZ-150), and Sclerotinia sclerotiorum botybirnavirus 3 (SsBV3/SZ-150) in four strains by RT-PCR. The actin gene served as an internal control. (B) Colony morphology and (D) growth rate of four individual strains. Four strains were grown on PDA for 15 days at 20^∘C prior to photography. (C,E) Virulence assays of the four strains on the detached leaves of rapeseed plants. Statistical analysis of the growth rate and lesion diameter of the four individual strains was conducted at the P < 0.01 level. The error bars indicate the SD from three sample means.