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. 2019 Jul 2;10:1451. doi: 10.3389/fimmu.2019.01451

Figure 1.

Figure 1

TORC2−/− DC display augmented glycolytic activity, glycolysis-dependent ATP production and viability compared to wild-type (WT) control (Ctrl) DC. Bone marrow-derived DC were generated from WT C57BL/6 Ctrl or TORC2DC−/− mice (TORC2−/− DC), with or without LPS stimulation, and analyzed using a Seahorse XFe96 Bioanalyzer for metabolic flux in real-time over 125 min. (A) Representative glycolysis stress test showing basal glycolysis (ECAR), glycolytic capacity and glycolytic reserve; n = 4 independent experiments, with at least 2 mice per experiment; one-way ANOVA Tukey's multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001. (B) ATP production by non-stimulated WT Ctrl DC or TORC2−/− DC. (C) ATP production by WT Ctrl DC or TORC2−/− DC stimulated with LPS for 1 h; one-way ANOVA Tukey's multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (D) Representative histogram of 7AAD viability dye staining. (E) Quantification of viability as percentage of live (7AAD−) cells; (B–E) n = 5 independent experiments, with at least 2 mice per experiment; one-way ANOVA Tukey's multiple comparisons test, **p < 0.01, ***p < 0.001, ****p < 0.0001. ATP, adenosine triphosphate; 2-DG, 2-deoxyglucose; ECAR, extracellular acidification rate; NS, not significant.