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. 2019 Jul 2;10:1451. doi: 10.3389/fimmu.2019.01451

Figure 2.

Figure 2

TORC2−/− DC exhibit enhanced respiration and spare respiratory capacity (SRC). Bone marrow-derived DC were generated from WT control (Ctrl) or TORC2DC−/− mice (TORC2−/− DC), then stimulated with LPS for 18 h, as indicated. The DC were analyzed using a Seahorse XFe96 Bioanalyzer for metabolic flux in real-time over 125 min with (1) oligomycin, (2) FCCP, and (3) Rot/AA injected at the times indicated to obtain control values. (A) Representative mitochondria stress test of non-stimulated or LPS-stimulated WT Ctrl DC or TORC2−/− DC. (B) Quantification of basal respiration, maximal respiration and spare respiratory capacity (SRC). SRC was calculated as the difference in OCR after addition of FCCP (2) and OCR before the addition of oligomycin (1); n = 5 independent experiments with at least 2 mice per experiment; one-way ANOVA Tukey's multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. FCCP, carbonyl cyanide 4 p-(trifluoromethoxy) phenylhydrazone; Rot/AA, rotenone/antimycin A.