Figure 2.

Decreased interaction between neutrophils and platelets in the peripheral blood of mice lacking Nox2. (A) A greater percentage of circulating neutrophils in WT mice display platelet markers as demonstrated by dual positivity for CD41 and CD45 by flow cytometry 24 h following induction of SIRS by peritoneal injection of zymosan, n = 8, ^*p < 0.05. (B) Using a microfluidic adhesion assay, there are more platelets per neutrophil in the peripheral circulation of WT mice both at baseline and after induction of SIRS, and the interaction is unchanged in WT mice. In contrast, neutrophils from gp91^phox−/y mice demonstrate fewer neutrophil-platelet interactions at baseline and the number of aggregates decreases further after SIRS induction, n ≥ 47 cells per condition, ^*p < 0.05, ^**p < 0.01, ^****p < 0.0001.