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. 2019 Feb 1;10(4):963–971. doi: 10.1111/jdi.13001

Figure 2.

Figure 2

Effect of temperature on the stability of the glutathione S‐transferase–glucokinase fusion protein. Stock enzyme solutions were diluted to 220 μg/mL by 50 mmol/L Tris/HCl, pH 8.0. (a) The enzyme solutions were incubated for 30 min at different temperatures ranging from 30 to 60°C and assayed at 30°C. (b) The enzyme solutions were incubated for different periods of time from 5 to 30 min at 47.5°C. Mean ± standard error of the mean for three independent enzyme preparations are shown for each case. WT, wild type.