Figure 5.
Effects of knockdown of Kbtbd11 on mature 3T3‐L1 adipocytes. (a) Triglyceride accumulation in Kbtbd11‐knockdown mature 3T3‐L1 adipocytes at 48 h after either of the two independent Kbtbd11 short hairpin ribonucleic acid (shRNA) adenoviral infections (shRNA#1 and shRNA#2) visualized using Oil Red O staining; (b) RNA was harvested at 48 h after adenoviral infection, and expression levels of Kbtbd11, adipocytes (Pparg and aP2) and inflammation markers (Tnfa and Il6), and lipogenic (Fasn) and proapoptotic genes (Bax and Bcl2) were measured using quantitative polymerase chain reaction; n = 3 per group, *P < 0.01 versus shLacZ.