Table 5.

Primer Sequences for the Simple, Multiplexed, PCR-Based Barcoding of DNA for Sensitive Mutation Detection Using Sequencing Assays

Assay name	Mutation	Primer sequences
BRAF_305	V600E	Forward: 5′-GGACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNNATGGGAAAGAGTGTCCCAAACTGATGGGACCCACTCCATCG-3′
		Reverse: 5′-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGC-3′
KRAS_329	G12D	Forward: 5′-GGACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNNATGGGAAAGAGTGTCCGCCTGCTGAAAATGACTGAATATAAACTTG-3′
		Reverse: 5′-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGCTGTATCGTCAAGGCACTCTT-3′
NRAS_881_3	Q61R	Forward: 5′-GGACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNNATGGGAAAGAGTGTCCTTGGTCTCTCATGGCACTGTACTCTTCT-3′
		Reverse: 5′-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTAACCTGTTTGTTGGACATACTGGATACAGC-3′
PIK3CA_234	H1047R	Forward: 5′-GGACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNNATGGGAAAGAGTGTCCCTGAGCAAGAGGCTTTGGAGTATTTCATG-3′
		Reverse: 5′-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCAATCCATTTTTGTTGTCCAGCCAC-3′