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. 2019 Jul;21(4):718–733. doi: 10.1016/j.jmoldx.2019.03.002

Figure 3.

Figure 3

Determination of the linear range of the test. Linearity of the assay was established by measuring a range spanning nearly five orders of magnitude across 288 valid measurements across two lots. The e13a2 and e14a2 breakpoints were evaluated and charted separately (A and B, respectively), with the x axis representing the targeted molecular response (MR) value of the specimen and the y axis representing the measured MR value. The symbols are depicted with 50% transparency to clarify overplotting. The 95% CI is shown in red around the largely overlapping, dotted linear regression line. The green dotted lines are drawn at the limit of detection of MR4.7. The linear regression spans MR0.1 to MR4.8, with the equations indicated for e13a2 and e14a2.