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. 2019 Jul;21(4):718–733. doi: 10.1016/j.jmoldx.2019.03.002

Figure 4.

Figure 4

RNA input range. The RNA input range of the assay was confirmed by measuring specimens at four distinct molecular response (MR) values with six total RNA input amounts, including three input amounts outside of the recommended input range: 250, 500, and 6000 ng. Dilution series labeled A, B, C, and D were targeted to MR1.0, MR3.0, MR4.3, and MR4.5, respectively. Actual values were determined on testing. The x axis represents total RNA amount included in the RT, and the y axis represents measured MR value (with highest BCR-ABL1 analyte level at the top); symbol colors indicate different specimens (depicted with 50% transparency to clarify overplotting); the gray region is the recommended input range (1000 to 5000 ng); and the green dotted line is the test's limit of detection of MR4.7.