Table 3.

LOD Data

Transcript identity	Unique specimens	Valid measurements	Positive results	Detected, %	LOD (MR)	LOD (%IS)
e13a2	3	179	172	96.1	4.70	0.002
e14a2	7	420	400	95.2	4.70	0.002

The LOD of the test was estimated using Probit analysis (Figure 2) and validated using the nonparametric method, wherein >95% of all low-level specimens targeted near the expected LOD of MR4.7 (≥0.002%IS) were detected. MR measurements formed the definitive acceptance criteria. %IS values are shown for historical reference.

IS, international scale; LOD, limit of detection; MR, molecular response.