Table 5.

RNA Isolation Method

Specimen no.	Isolation	Mean MR	Median MR	SD	n
1	Kingfisher	0.61	0.62	0.06	16
1	RNeasy	0.62	0.64	0.07	16
1	TRIzol	0.73	0.75	0.05	16
1	All	0.66	0.67	0.08	48
2	Kingfisher	1.48	1.48	0.03	16
2	RNeasy	1.47	1.47	0.02	16
2	TRIzol	1.54	1.55	0.03	16
2	All	1.50	1.49	0.04	48
3	Kingfisher	2.49	2.47	0.04	16
3	RNeasy	2.48	2.48	0.03	16
3	TRIzol	2.56	2.55	0.03	16
3	All	2.51	2.50	0.05	48
4	Kingfisher	3.52	3.51	0.07	16
4	RNeasy	3.47	3.46	0.07	16
4	TRIzol	3.56	3.56	0.08	16
4	All	3.52	3.51	0.08	48

Freshly drawn, enriched, human white blood cells from a chronic myeloid leukemia (CML)–positive donor were serially diluted across four logs into CML-negative anticoagulated whole blood, generating specimens 1, 2, 3, and 4. The resulting specimens were subjected to RNA extraction by three methods: TRIzol guanidinium thiocyanate-phenol-chloroform extraction with isopropanol precipitation, the column-based RNeasy Mini Kit (Qiagen), and an automated, customized magnetic bead–based RNA isolation (Kingfisher Flex using RNAClean XL magnetic beads). All three isolation methods were evaluated for similarity by assessing the variability of all MR values per specimen across the three methods against the acceptable precision of the method. Shown are MR values (mean and median), SD, and number of valid measurements (n). All refers to the aggregate data of all three isolation methods within a specimen.

MR, molecular response.