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. 2019 Feb 25;10(4):1108–1111. doi: 10.1111/jdi.12997

Figure 2.

Figure 2

(a)The frequencies of cytokine‐producing CD8+ T cells in response to islet antigen peptide clusters, glutamic acid decarboxylase (GAD)65‐C1, preproinsulin (PPI)‐C2, islet‐specific glucose‐6‐phosphatase catalytic subunit‐related protein (IGRP)‐C3 and zinc transporter‐8 antibody (ZnT8‐C4), with peptide diluent as a negative control in the patient with type 1 diabetes, presenting with idiopathic CD4 lymphocytopenia, and a non‐diabetic individual. Intracytoplasmic cytokine detection assay was carried out after 7‐day stimulation with islet antigen peptide clusters in the presence of interleukin‐2 (IL‐2), gated to LIVE/DEAD − CD3+ CD8+ T‐cell populations. (b)Comparison of the frequencies of islet‐specific glucose‐6‐phosphatase catalytic subunit‐related protein (IGRP)‐C3‐specific interferon‐gamma (IFN‐γ)‐producing CD8+ T cells between the patient and non‐diabetic individuals (n = 15). CD4, CD4+ T cells; CD8, CD8+ T cells; IL‐10, interleukin‐10; IFN‐γ, interferon‐gamma; Pt, patient with type 1 diabetes, presenting with idiopathic CD4 lymphocytopenia; ND, non‐diabetic individual.