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. 2019 Jun 6;13(1):163–176. doi: 10.1016/j.stemcr.2019.05.005

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© 2019 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Control and Mutant Lines and VUB03_BcL-xL Equally Differentiate to Mesendoderm Derivatives

(A) Representative examples of immunostaining for T (green) and POU5F1 (red) in control (VUB01_wt) and mutant (VUB01_mt) lines after mesendoderm induction.

(B) Percentage of T/POU5F1 double-positive (mesendoderm cells) and POU5F1-only positive cells.

(C) Examples of immunostaining for SOX17 (green) and POU5F1 (red) in control (VUB01_wt and VUB03_wt) and mutant (VUB01_wt and VUB03_mt) lines, and VUB03_BcL-xL after definitive endoderm differentiation.

(D) Percentage of SOX17- and POU5F1-positive cells.

(E) Relative mRNA expression as measured by qPCR (n = 3 to 6). Data are shown as means ± SEM, each dot represents an independent differentiation experiment and the horizontal bars with asterisks represent statistical significance between samples (p < 0.05, t test).