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. 2019 Jun 6;13(1):163–176. doi: 10.1016/j.stemcr.2019.05.005

Figure 6.

Figure 6

Knockdown and Overexpression of CHCHD2 Do Not Affect Neuroectoderm Differentiation

(A) CHCHD2 expression as measured by qPCR in VUB01_wt 24, 48, and 72 h after transfection with siRNA against CHCHD2 (n = 1).

(B) Representative immunostaining of untreated and transfected cells 72 h posttransfection (the CHCHD2 protein can be seen in green).

(C and D) CHCHD2 (C) and PAX6 and SOX1 (D) expression as measured by qPCR in VUB01 WT during a 4-day induction to neuroectoderm. NT, nontargeting siRNA (n = 1).

(E) Immunostaining of untreated cells and after 72 h of exogenous CHCHD2 expression (in green).

(F) PAX6 and SOX1 expression as measured by qPCR in VUB01_mt during a 4-day induction to neuroectoderm in untreated, control (GFP) and CHCHD2-overexpressing cells (n = 1).