Figure 3.
DPY30 Deficiency Impairs Mitochondrial Function and Alters Quiescence State of the BM HSCs
BMs from pIpC-injected Mx1-Cre; Dpy30F/+ (F/+) and Mx1-Cre; Dpy30F/− (F/−) mice were used in this figure.
(A and B) Oxygen consumption rate (OCR) (A) and basal extracellular acidification rates (B) of live Lin− BM cells. n = 3 mice each.
(C, E, and F) Analyses (left) and representative histograms (right) for mitochondrial membrane potential (C), mitochondrial mass (E) and ROS level (F) of indicated BM cell populations. n = 3 mice each.
(D) Mitochondrial DNA levels from BM LSK cells were determined by qPCR on mt-Co2 and normalized to Hbb for genomic DNA levels. n = 3 mice each.
(G and H) Total cellular ATP levels (G), and NAD+/NADH and NADP+/NADPH ratios (H) in BM LSK cells were determined. n = 3 mice each.
(I) Representative FACS plots of Ki-67 staining on LT-HSCs.
(J) G0, G1, and S/G2/M phases of LT-HSCs were quantified. n = 5 mice each. Data are shown as mean ± SD for all panels. n.s., not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, by two-tailed Student's t test.
See also Figure S4.