Figure 5.

Inhibition of DDR Partially Rescues the Function of the DPY30-Deficient Hematopoietic Cells

(A–E) Lin⁻ BM cells from CAG-CreER; Dpy30^F/F mice were treated with indicated agents in liquid culture for 2 days and subjected to colony formation assays.

(A) Relative Dpy30 mRNA levels were determined by qRT-PCR and normalized to Actb. n = 3 each.

(B and C) All colonies (B) (three independent biological repeats) and different types of colonies (C) (two independent biological repeats) were quantified.

(D and E) Representative image of the plates (D) and colonies (E) are shown.

(F–H) Mx1-Cre; Dpy30^F/+ or Mx1-Cre; Dpy30^F/− mice were injected with pIpC and either vehicle (DMSO) or ATM inhibitor KU55933 (F), followed by determination of mitochondrial membrane potential of HSCs by FACS. The FACS plots with the gating for the effect of KU55933 in the KO mice are shown in (G) where individual mouse is represented by the individual line, and the results are quantified in (H), n = 3 for each.

Data are shown as mean ± SD for all bar graphs. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, by one-factor ANOVA with post hoc t test. Scale bars, 400 μm. See also Figure S6.