Figure 2.

Effect of Transduction with Reprogramming Factors on Senescence and Cell Proliferation

(A and B) Primary (A) and metastatic (B) melanoma cells senescence (red lines) and survival/proliferation (green lines). Data (mean ± SD; n = 3 replicate wells/cell line for each time point) are shown. Approximately 5,000 cells/well of 24-well plates were seeded and transduced with reprogramming factor lentiviruses (day 0) and all wells were scanned using an EVOS FL Auto microscope, and cell number and percent SA-β-gal-stained cells were estimated using ImageJ analysis of the scanned images.

(C and D) Western blot analysis of p21 expression at 1 and 5 days after transduction in primary (C) and metastatic cells (D) and at miPSC stage. GAPDH shows equal loading.