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. 2019 Jun 20;13(1):177–192. doi: 10.1016/j.stemcr.2019.05.018

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© 2019 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Melanocyte Differentiation of Fibroblast-iPSCs, Melanocyte-iPSCs, and Melanoma-iPSCs

(A) Morphology of parental and iPSCs induced to differentiate in melanocyte medium. Top–bottom: cells differentiated from fibroblast-iPSCs, melanocyte-iPSCs, and primary melanoma-iPSCs: WM115, WM1552C, WM1361A, and WM1862; and metastatic iPSCs: MRA2 and MRA5.

(B) Cell pellets from parental melanoma and miPSC-differentiated cells.

(C) Reprogramming of human melanocytes expressing NICD-GFP, mouse fibroblasts, and BRAF^V600/Pten^−/– mouse tumor cells, EB formation, and melanocyte differentiation.

(D) Immunofluorescence staining of cells differentiated from fibroblast-iPSCs in melanocyte differentiation medium (top panels) and neuronal differentiation medium (bottom panels) with melanocytic markers SOX10 and MITF, and neuronal markers, TUJ1 and SYN1.

(E and F) Immunofluorescence of cells differentiated from primary WM1862 (E) and metastatic MRA5 (F) melanoma miPSCs in melanocyte differentiation medium with melanocytic marker MITF and neural markers GFAP and MAP2.