PCR analysis of plants with obvious albino phenotypes using the Bar gene and vector-specific primers. A, C and E PCR results for the Bar gene (433 bp) of Lilium pumilum DC. Fisch. G
Bar gene (433 bp) of ‘White Heaven’. B, D and F PCR fragment of a partial sgRNA expression cassette (831 bp) of Lilium pumilum DC. Fisch. H PCR fragment of partial sgRNA expression cassette (831 bp) of ‘White Heaven’. Lane M, DL2000 DNA marker. lane WT, wild-type plant. lane P, plasmid control. A–F: Lanes 1–45, obvious albino phenotypes plants of Lilium pumilum DC. Fisch. G and H: Lanes W1–16, obvious albino phenotypes plants of ‘White Heaven’, Only 16 lines are shown, and others no band was amplified.