Skip to main content
. 2019 Jun 14;20(12):2920. doi: 10.3390/ijms20122920

Table A1.

Media used for Lilium explant transformation.

Media Composition
Lilium pumilum DC. Fisch.
Pre-cultivation I MS (Murashige-Skoog) + 1.0 mg·L−1 PIC (Picloram) + 0.2 mg·L−1 NAA (Naphthylacetic acid) + 30 g·L−1 Sucrose + 7.0 g·L−1 Agarose
Co-cultivation I MS (NH4NO3-free) + 1.0 mg·L−1 PIC + 0.2 mg·L−1 NAA +100 μmol AS (Acetosyringone) + 60 g·L−1 Sucrose + 7.0 g·L−1 Agarose
Selection I MS + 1.0 mg·L−1 PIC + 0.2 mg·L−1 NAA + 400 mg·L−1 Cef (Cefotaxime) + 30 mg·L−1 Hyg (Hygromycin) + 30 g·L−1 Sucrose + 7.0 g·L−1 Agarose
Selection II MS + 1.0 mg·L−1 PIC + 0.2 mg·L−1 NAA + 400 mg·L−1 Cef + 10 mg·L−1 Basta +30 g·L−1 Sucrose + 7.0 g·L−1 Agarose
Somatic embryo germination medium MS + 0.5 mg·L−1 6-BA (N6-benzyladenine) + 30 g·L−1 Sucrose + 7.0 g·L−1 Agarose
‘White Heaven’
Pre-cultivation II MS + 1.5 mg·L−1 BA + 0.2 mg·L−1 NAA + 30 g·L−1 Sucrose + 7.0 g·L−1 Agarose
Co-cultivation II MS (NH4NO3-free) + 1.5 mg·L−1 BA + 0.2 mg·L−1 NAA +100 μmol AS + 60 g·L−1 Sucrose + 7.0 g·L−1 Agarose
Selection III MS (NH4NO3-free) + 1.5 mg·L−1 BA + 0.2 mg·L−1 NAA + 400 mg·L−1 Cef + 40 mg·L−1 Hyg +30 g·L−1 Sucrose + 7.0 g·L−1 Agarose
Selection IV MS (NH4NO3-free) + 1.5 mg·L−1 BA + 0.2 mg·L−1 NAA + 400 mg·L−1 Cef + 20 mg·L−1 Basta + 30 g·L−1 Sucrose + 7.0 g·L−1 Agarose
Resuspension solution ½MS (NH4NO3-free) + 100 μmol AS + 60 g·L−1 Sucrose