Lilium pumilum DC. Fisch. |
Pre-cultivation I |
MS (Murashige-Skoog) + 1.0 mg·L−1 PIC (Picloram) + 0.2 mg·L−1 NAA (Naphthylacetic acid) + 30 g·L−1 Sucrose + 7.0 g·L−1 Agarose |
Co-cultivation I |
MS (NH4NO3-free) + 1.0 mg·L−1 PIC + 0.2 mg·L−1 NAA +100 μmol AS (Acetosyringone) + 60 g·L−1 Sucrose + 7.0 g·L−1 Agarose |
Selection I |
MS + 1.0 mg·L−1 PIC + 0.2 mg·L−1 NAA + 400 mg·L−1 Cef (Cefotaxime) + 30 mg·L−1 Hyg (Hygromycin) + 30 g·L−1 Sucrose + 7.0 g·L−1 Agarose |
Selection II |
MS + 1.0 mg·L−1 PIC + 0.2 mg·L−1 NAA + 400 mg·L−1 Cef + 10 mg·L−1 Basta +30 g·L−1 Sucrose + 7.0 g·L−1 Agarose |
Somatic embryo germination medium |
MS + 0.5 mg·L−1 6-BA (N6-benzyladenine) + 30 g·L−1 Sucrose + 7.0 g·L−1 Agarose |
‘White Heaven’ |
Pre-cultivation II |
MS + 1.5 mg·L−1 BA + 0.2 mg·L−1 NAA + 30 g·L−1 Sucrose + 7.0 g·L−1 Agarose |
Co-cultivation II |
MS (NH4NO3-free) + 1.5 mg·L−1 BA + 0.2 mg·L−1 NAA +100 μmol AS + 60 g·L−1 Sucrose + 7.0 g·L−1 Agarose |
Selection III |
MS (NH4NO3-free) + 1.5 mg·L−1 BA + 0.2 mg·L−1 NAA + 400 mg·L−1 Cef + 40 mg·L−1 Hyg +30 g·L−1 Sucrose + 7.0 g·L−1 Agarose |
Selection IV |
MS (NH4NO3-free) + 1.5 mg·L−1 BA + 0.2 mg·L−1 NAA + 400 mg·L−1 Cef + 20 mg·L−1 Basta + 30 g·L−1 Sucrose + 7.0 g·L−1 Agarose |
Resuspension solution |
½MS (NH4NO3-free) + 100 μmol AS + 60 g·L−1 Sucrose |