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Mitochondrial membrane potential variation following 1 h and 3 h of treatment. (a) Confocal microscopy images of cells treated with TMAO 100 µM and TMAO 10 mM for 1 h and 3 h and labeled with the JC-1 probe (60× magnification). (b) Bar graph showing red/green fluorescence after 1 h and 3 h of treatment, no variations of mitochondrial membrane potential are detected (refer to the main text for numerical values).
