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. 2019 Jun 8;11(6):797. doi: 10.3390/cancers11060797

Figure 5.

Figure 5

MPPED2 reduces cell proliferation of breast carcinoma cell lines. (A,B) qRT-PCR (left panel) performed in MDA-MB-231 and SKBR3 cells stably expressing MPPED2 or carrying the corresponding empty vector (EV). Data are reported as 2−ΔΔCt values ± SD, compared to the EV, set equal to 1. Western bot (right panel) analysis confirming the expression of MPPED2. β-actin was used to normalize the amount of loaded protein (left panel). Densitometric analysis was performed by using ImageJ software to evaluate MPPED2 protein expression compared to EV, set equal to 1. Original blots were shown in Supplementary Materials Figure S5. (C,D) Cell growth analysis of MDA-MB-231 and SKBR3 cells stably expressing MPPED2 or carrying the corresponding empty vector (EV). Cell number was evaluated at 24 h, 48 h, 72 h and 96h after seeding. Values were obtained from three independent experiments performed in duplicate. Data are reported as mean ± SD. 2-way ANOVA test (Bonferroni post-test: MPPED2 vs. EV, 96 h and 72 h, ***, p < 0.001). (E,F) Representative colony assays performed in MDA-MB-231 and SKBR3 cells transiently transfected with MPPED2 or the corresponding empty vector (EV). Cell were stained with crystal violet after 3 weeks of selection with hygromycin.