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. 2019 Jun 8;11(6):797. doi: 10.3390/cancers11060797

Figure 6.

Figure 6

MPPED2 inhibits cell cycle progression of breast carcinoma cell lines. (A,B) Representative experiments of cell cycle analysis performed in MDA-MB-231 and SKBR3 cells stably transfected with MPPED2 or the corresponding empty vector (EV) are shown in left panel. Values shown in the right panel were obtained from three independent experiments. t-test: *, p = 0.0286 (MPPED2 vs. EV, MDA-MB-231 G1 phase); **, p = 0.0079 (MPPED2 vs. EV, SKBR3 G1 phase). (C) Western blot analysis of cyclin D and cyclin E expression in MD-MBA-231 (left panel) and SKBR3 (right panel) stably expressing MPPED2 or carrying the corresponding empty vector (EV). β-actin was used to normalize the amount of loaded protein. Densitometric analysis was performed by using ImageJ software to analyse protein expression compared to the EV, set equal to 1. Original blots were shown in Supplementary Materials Figure S6.