Figure 22.

Silver triangular nanoparticles fabricated by NSL on a glass substrate. (A) Tapping mode AFM image of the Ag triangular NPs. (B) Surface chemistry of the Ag nanobiosensor. A mixed monolayer of (1) 11-MUA and (2) 1-OT is formed on the exposed surfaces of the AgNPs followed by the covalent linking of (3) biotin to the carboxyl groups of (1) 11-MUA. Schematic illustration of (C) streptavidin binding to a biotinylated Ag nanobiosensor and (D) biotin covalently linked to the Ag nanobiosensor surface while antibiotin-labeled AuNPs are subsequently exposed to the surface. LSPR spectra (E) before (solid black) and after (dashed blue) binding of native antibiotin and (F) before (solid black) and after (dashed red) binding of antibiotin-labeled NPs. Adapted from [57,177]. Copyright (2002, 2011), American Chemical Society.