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. 2019 Jun 19;10(6):467. doi: 10.3390/genes10060467

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The transformation efficiency as affected by Agrobacterium tumefaciens strains and acetosyringone (AS) concentration. (A) The hygromycin; (B) resistance phenotype of transformation in the 1st, 2nd, and 3rd round of selection when using EHA105 and AGL1, respectively; (C) The hygromycin-B-resistant phenotype of transformation in the 2nd and 3rd round of selection when using AS at different concentrations (200 µM, 100 µM, and 0 µM); (D) The number of hygromycin-B-resistant colonies per plate in the second selection. MYG: malt extract, yeast extract and glucose (MYG) agar medium.