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PCR analysis of L. edodes transformants. The genomic DNA of the potential transformants was subjected to PCR amplification of eGFP contained in the T-DNA regions. The size of the expected PCR product is indicated on the right. Lanes: M: 1kb DNA Ladder (TSINGKE, Beijing, China); 1–21: Different hygromycin-resistant transformants after the third selection; 22: Untransformed W1; 23: Plasmid DNA; 24: ddH2O.
