Table 3.
Agrobacterium-mediated transformation (ATMT) transformation efficiency of different L. edodes variants.
| Testing Item | Variety | ||||
|---|---|---|---|---|---|
| W1 | S606 | YS3357 | YS3334 | YS55 | |
| 1st (%) a | 12.26 ± 2.04 | 1.41 ± 0.40 | 1.97 ± 0.41 | 7.27 ± 2.94 | 32.96 ± 10.82 |
| 2nd b | 7.00 ± 4.69 | 49.50 ± 4.43 | 79.00 ± 8.83 | 85.25 ± 8.54 | 61.00 ± 16.02 |
| PF (%) c | 2.79 ± 3.23 | 18.86 ± 8.10 | 26.98 ± 14.55 | 85.12 ± 14.31 | 34.47 ± 16.62 |
a Mean germination frequency per assay ± SE in the first selection. b Mean number of hygromycin-B-resistant colonies per plate ± SE in the second selection. c Mean positive frequency of PCR analysis per assay ± SE after the third selection.