Table 1.

Primers for polymerase chain reaction (PCR)/Sanger sequencing.

GJB2	Primers	Reference
GJB2 coding region (exon 2) and flanking sequences	835-F: 5’-TGCTTGCTTACCCAGACTCA-3’ 835-R: 5’-CCTCATCCCTCTCATGCTGT-3’	This study
	or
	F3044: 5’-AGTGCCTTTCAGCTAACGA-3’ R4242: 5’-GTGGCATCTGGAGTTTCACC-3’
Upstream region of exon 1 (which includes the basal promoter), exon 1, donor splice site, and flanking intronic region	Ex1-F: 5’-TCTTTTCCAGAGCAAACCGC-3’ Ex1-R: 5’-CTGGGCAATGCGTTAAACTGG-3’	[30]
	Ex1-792-F: 5’-GCGTTCGTTCGGATTGGT-3’ Ex1-2239-R: 5’-CGGAAACAGACCCTCGTGAAGT-3’	This study
	PF1: 5’- GGCTCAAAGGAACTAGGAGATCG-3’ PF2: 5’-CGTTCGTTCGGATTGGTGAG-3’PR1: 5’-CAGAAACGCCCGCTCCAGAA-3’	[29]

F: Forward; R: Reverse.