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. 2019 Jun 14;11(6):1343. doi: 10.3390/nu11061343

Figure 3.

Figure 3

Melatonin attenuates IL-1β-induced ICAM-1 and VCAM-1 expression in GBM. U251 and U87 cells were pretreated with various concentration of melatonin (0.25, 0.5 or 1 mM) for 45 min, then added to IL-1β (3 ng/mL) for another 24 h (A), or 6 h (C). ICAM-1 and VCAM-1 expression were analyzed by western blotting (A) and real-time PCR (C), respectively. (B) U251 and U87 cells were pretreated with melatonin (0.5 or 1 mM) for 45 min then added with IL-1β (3 ng/mL) for another 24 h. The binding activity of monocyte were analyzed by evaluating the BCECF-AM-labeled-THP-1 monocytes by the fluorescence microscopy. The data are presented as mean ± S.E.M. (representative of independent experiments = 3). * p < 0.05 compared with the control group. # p < 0.05 compared with the IL-1β treatment group.