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. 2019 May 29;11(6):747. doi: 10.3390/cancers11060747

Figure 2.

Figure 2

Effect of HDACis on C6 cells ability to form vascular tubes. (A) Representative images of vascular tubes formed by C6 cells in control group (DMSO 0.1%) and in treated group. All HDACis are used at final concentration of 50 nM. Network of tubes, in each well, were analyzed directly under an inverted microscope with 5× phase contrast and imaged. Scale bars: 250 µm. (B) Quantification of vascular branching. Branch point (sites of intersection of at least three tubes) number in each well was counted and expressed as a percentage of branch points formed in the control sample, taken as 100%. As shown, SAHA and MC1568 reduce C6 ability to form vascular tubes, whereas TSA and MS275 have no effect. (C) The cell rate viability assayed by MTT assay. At concentration of 50 nM, no differences in 24 h was detected in treated groups as compared to the control group. *** p-value < 0.001.