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. 2019 Jun 8;11(6):1303. doi: 10.3390/nu11061303

Figure 4.

Figure 4

Effect of leek extract on the bronchial permeability to Ole e 1. (A) Time course of TEER values on day 2 ALI-cultured Calu-3 cells after treatment with leek extract (LE) compared to control cells (PBS). Data are the mean ± SD of triplicate determinations. * p < 0.05. (B) ALI-cultured Calu-3 cells were exposed to Ole e 1 (2.5 µg) on day 2 for 7 h after treatment with leek extract (LE, 5 μg/mL) for 24 h, and the presence of the allergen was determined in the medium using a rabbit polyclonal antibody. (C) ALI-cultured Calu-3 cells were treated with leek extract at 5 μg/mL (LE) for 24 h on day 2, and IL-6 levels in the medium were determined by sandwich ELISA. PBS, control cells. Data are the mean ± SD of triplicate determinations ** p < 0.01.