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. 2019 Jun 25;20(12):3107. doi: 10.3390/ijms20123107

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Endoglin expression in GM-MΦ and M-MΦ. Human monocytes were incubated with GM-CSF or M-CSF, resulting in GM-MΦ and M-MΦ, respectively. Soluble endoglin (sEng) levels were measured by ELISA in culture supernatants at the final stage of differentiation (A) or during a kinetic study (B). In panel B, the statistical significance with respect to the control sample (day 0) is indicated. (C) Comparative mRNA expression levels of endoglin along GM-MΦ and M-MΦ polarization, as determined by qRT-PCR. (D) Endoglin protein levels at the surface of GM-MΦ and M-MΦ were detected by immunofluorescence flow cytometry using a mouse monoclonal antibody directed against human endoglin. Values of mean fluorescence intensity (MFI) are indicated. Statistical analysis was calculated using the paired two-tailed Student t test (n = 3), and data are presented as mean ± SD. (*p < 0.05; **p < 0.01).