Figure 7.

MMP-12-dependent release of soluble endoglin in mice. (A) Transcript expression levels of Mmp-12 in murine bone marrow-derived MΦ incubated with GM-CSF (GM-MΦ) or M-CSF (M-MΦ), as determined by qRT-PCR. Statistical significance of data (n = 4) was calculated using a paired, two-tailed Student t test, and data are presented as mean ± SD. (B) Soluble endoglin levels in lipopolysaccharide (LPS)-treated animals. Mice were injected (i.p.) with LPS and orally gavaged with MMP-408 or vehicle (PBS). Plasma samples were extracted, and soluble endoglin was measured using an ELISA kit. A point cloud representation is shown where blue horizontal bars indicate the median values of each condition. Statistical significance was calculated (n ≥ 5) using the ANOVA test, and data are presented as mean ± SD.