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. 2019 Jun 14;20(12):2923. doi: 10.3390/ijms20122923

Figure 6.

Figure 6

IGF2BP1 promotes chicken adipocyte differentiation and increases lipid droplet accumulation. (a) The expression of IGF2BP1 during ICP cell differentiation. (b) The expression of genes related to adipocyte differentiation and fatty acid metabolism after IGF2BP1 overexpression in an ICP cell. (c). The expression of genes related to adipocyte differentiation and fatty acid metabolism after inhibition of IGF2BP1 expression in an ICP cell. (d). Representative images of oil red O staining (red) after overexpression of IGF2BP1 in an ICP cell; scale bar: 100 μm. (e). Lipid droplet content by oil red O staining and extraction method of cells transfected with IGF2BP1 overexpression vector. (f). Representative images of oil red O staining (red) after inhibition of IGF2BP1 in an ICP cell; scale bar: 100 μm. (g). Lipid droplet content by oil red O staining and extraction method of cells transfected with si-IGF2BP1 and si-NC. The data are mean ± S.E.M. with four samples (n = 4/treatment group). Independent sample t test was used to analyze the statistical differences between groups. * p < 0.05; ** p < 0.01.