Table 2.

Pooling of sera and plasma for IgE-western blot and used dilution. Membrane number indicates the membrane that was incubated with the specific pooled specimen.

Pool/Serum	Patient Serum/Plasma	Dilution
Pool 1	7, 11, and 12	1:5
Serum 6	6	1:7
Pool 2	8 and 9	1:7
Pool 3	1 and 10	1:5