Table 2.
The estimated secondary structure content (%) of Ranatuerin-2Pb, RPa and RPb from obtained CD spectra in different solutions using BESTSEL CD spectrum analysis tool.
| Solution | Peptide | Helix | Antiparallel | Parallel | Turn | Others |
|---|---|---|---|---|---|---|
| 50% TFE/ H2O | Ranatuerin-2Pb | 50 | 7.8 | 1.2 | 10.9 | 29.8 |
| RPa | 33.6 | 18.1 | 4.3 | 11.4 | 32.5 | |
| RPb | 49.6 | 7.6 | 0 | 9.8 | 33 | |
| H2O | Ranatuerin-2Pb | 3.5 | 26.6 | 0 | 18.3 | 51.5 |
| RPa | 2.4 | 30.6 | 0 | 17.3 | 49.7 | |
| RPb | 0.3 | 25.4 | 0 | 19.4 | 55 | |
| POPC/POPG 1:1 | Ranatuerin-2Pb | 59.2 | 1.2 | 2.7 | 10.8 | 26.1 |
| RPa | 24.9 | 13.7 | 8.6 | 13.7 | 39.1 | |
| RPb | 51.8 | 3.6 | 0 | 12.2 | 32.4 | |
| POPE/POPG 3:1 | Ranatuerin-2Pb | 40 | 3.8 | 8.7 | 11.8 | 35.7 |
| RPa | 23.6 | 24.4 | 6.9 | 12.1 | 33 | |
| RPb | 51.6 | 9.2 | 0 | 12 | 27.2 |