Figure 3.

Effect of IDH1^R132H expression on the level of glutathione (GSH) in glioma cells. GSH level in stably transduced U-251MG (a), U-343MG (b), and LN-229 (c) cells after irradiation with 0 or 5 Gy. Total GSH levels (sum of reduced GSH and oxidized GSSG) were set as 100%. Bars represent the mean values of four independent experiments. Error bars indicate standard deviations (±SD). UT: untreated, pLVX: cells stably transduced with empty vector, pLVX IDH1^wt: IDH1^wt-expressing cells, pLVX IDH1^R132H: IDH1^R132H-expressing cells. ** p < 0.01 (compared to the respective IDH1^wt cells).