Figure A1.

Effect of IDH1^R132H gene expression on the accumulation of residual γH2AX foci after radiation in U-343MG cells. Representative immunofluorescence images of γH2AX of U-343MG cells 24 h after irradiation with 2 (a,b) and 4 Gy (c, d) under normoxia (a,c) and hypoxia (b,d). Green: γH2AX foci; Blue: Cell nuclei (DAPI). n = 3 independent experiments; scale bar = 25 µm. Normoxia (21% O₂), hypoxia (< 0.1% O₂). UT: untreated, pLVX: cells stably transduced with empty vector, pLVX IDH1^wt: IDH1^wt-expressing cells, pLVX IDH1^R132H: IDH1^R132H-expressing cells.