Figure A6.

Effect of IDH1^wt or IDH1^R132H on mRNA expression of actin-regulating proteins. mRNA expression levels (qPCR) of RAC1 (a), RHOA (b), RHOB (c), RHOC (d), and CDC42 (e) in stably transduced U-251MG, U-343MG, and LN-229 cells. Bars represent the mean values, relative to the control (empty vector pLVX set as 100%), of three independent experiments. Error bars indicate standard deviations (±SD). UT: untreated cells, pLVX: cells stably transduced with empty vector, pLVX IDH1^wt: IDH1^wt-expressing cells, pLVX IDH1^R132H: IDH1^R132H-expressing cells. * p < 0.05 and ** p < 0.01 (compared to the respective IDH1^wt cells).