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. 2019 Jun 17;38(14):e100978. doi: 10.15252/embj.2018100978

Figure 1. RNF34 negatively regulates RLR signaling pathways.

Figure 1

  • A, B
    Results of the ELISAs used to determine IFN‐β (A) and IL6 (B) secretion in PMA‐differentiated THP‐1 cells transfected with siCtrl, siRNF34‐1, siRNF34‐2, or siRNF34‐3 oligos and infected with VSV for the indicated times.
  • C, D
    Luciferase activity driven by the IFN‐β (C) or NF‐κB (D) promoter in HEK293T cells transfected with Flag‐RNF34 or its E3 ligase‐dead mutant H342A followed by VSV infection for the indicated times. Luciferase assays were performed 24 h after transfection, and luciferase activity was reported as the fold induction.
  • E
    Plaque assay of VSV loads in supernatants from cells transfected with shCtrl, shRNF34‐1, shRNF34‐2, or shRNF34‐3 and subsequently infected with VSV.
  • F
    Microscopy images of shCtrl, shRNF34‐1, shRNF34‐2, or shRNF34‐3 cells infected with NDV‐GFP (MOI = 1.0) for the indicated times. Gray represents cells; green represents NDV. Scale bar, 300 μm.
  • G
    Immunoblot showing the levels of the phosphorylated (p) and total IRF3, TBK1, and VSV‐G proteins in PMA‐differentiated THP‐1 cells transfected with siCtrl or siRNF34‐1 oligos and infected with VSV for the indicated times.
  • H, J
    RT–PCR analysis of the expression of the IFN‐β (H), IL8, ISG54, and ISG56 (J) mRNAs in PBMCs transfected with siCtrl or siRNF34‐1 oligos and infected with VSV for the indicated times. The results were normalized to the values obtained prior to VSV infection.
  • I
    Results of the ELISA used to determine IFN‐β secretion in PBMCs transfected with siCtrl or siRNF34‐1 oligos and infected with VSV for the indicated times.
Data information: Cell‐based studies were performed independently at least three times with comparable results.Cell‐based studies were performed independently at least three times with comparable results. The luciferase reporter and ELISA data are presented as means ± SEM. Two‐tailed Student's t‐test was used for statistical analyses: *P < 0.05; **P < 0.01; and ***P < 0.001.Source data are available online for this figure.